6SM5
MbC/SHP1-C-SH2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-10 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.898, 63.820, 73.101 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.100 - 2.750 |
| R-factor | 0.224949000386 |
| Rwork | 0.219 |
| R-free | 0.27645 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jeg |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.618 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.100 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmeas | 0.140 | 0.650 |
| Number of reflections | 10640 | 1726 |
| <I/σ(I)> | 7.09 | 1.73 |
| Completeness [%] | 92.0 | |
| Redundancy | 2.11 | |
| CC(1/2) | 0.998 | 0.688 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M sodium citrate tribasic, 0.1 M bistros propane 8.4 and 20 % PEG 3350 |
