6SLH
Conformational flexibility within the small domain of human serine racemase.
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-18 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.200, 155.740, 85.580 |
Unit cell angles | 90.00, 98.48, 90.00 |
Refinement procedure
Resolution | 40.870 - 1.890 |
Rwork | 0.172 |
R-free | 0.21580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hmk |
RMSD bond length | 0.011 |
RMSD bond angle | 1.859 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.870 | 1.920 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmeas | 0.071 | 1.086 |
Number of reflections | 98693 | 4996 |
<I/σ(I)> | 10.5 | 1.5 |
Completeness [%] | 99.2 | |
Redundancy | 3.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100 mM Bis-Tris pH 6.5, 15% PEG 3350, 250 mM MgCl2 |