6SK8
DeltaC3 C-terminal truncation of HsNMT1 in complex with MyrCoA and GDCFSKPR substrates
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.978565 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.622, 58.256, 154.789 |
| Unit cell angles | 90.00, 91.26, 90.00 |
Refinement procedure
| Resolution | 49.310 - 1.870 |
| R-factor | 0.2096 |
| Rwork | 0.208 |
| R-free | 0.24390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5o9v |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.691 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER (2.6.0) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.310 | 49.310 | 1.910 |
| High resolution limit [Å] | 1.870 | 8.970 | 1.870 |
| Rmerge | 0.159 | 0.061 | 1.219 |
| Rmeas | 0.171 | 0.067 | 1.307 |
| Rpim | 0.063 | 0.026 | 0.467 |
| Total number of observations | 507596 | 4649 | 33696 |
| Number of reflections | 68387 | 667 | 4360 |
| <I/σ(I)> | 7.3 | 19.8 | 1.6 |
| Completeness [%] | 99.9 | 99.3 | 100 |
| Redundancy | 7.4 | 7 | 7.7 |
| CC(1/2) | 0.994 | 0.995 | 0.665 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 19% PEG8K, 100mM Sodium Citrate pH 5.6, 100mM MgCl2, 100mM NaCl |
