6SK2

HsNMT1 in complex with both MyrCoA and Acetylated-GKSFSKPR peptide reveals N-terminal Lysine Myristoylation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.984002
Spacegroup name	P 21 21 2
Unit cell lengths	78.818, 178.442, 58.271
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.788 - 1.900
R-factor	0.174280163469
Rwork	0.172
R-free	0.21999
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5o9t
RMSD bond length	0.006
RMSD bond angle	0.858
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER (2.6.0)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.790	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.102	0.941
Rmeas	0.106	0.975
Rpim	0.028	0.252
Number of reflections	131286	4177
<I/σ(I)>	15.4
Completeness [%]	99.9	99.7
Redundancy	14.2	14.6
CC(1/2)	0.999	0.934

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	22% PEG8K, 100mM Sodium Citrate pH 5.6, 100mM MgCl2, 100mM NaCl