6SJD

ZC3H12B-ribonuclease domain bound to RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-16
Detector	PSI PILATUS 6M
Wavelength(s)	0.97626
Spacegroup name	P 43 21 2
Unit cell lengths	114.237, 114.237, 165.268
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.954 - 3.290
R-factor	0.2371
Rwork	0.235
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v33
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	93.972	93.972	3.532
High resolution limit [Å]	3.290	10.198	3.291
Rmerge	0.436	0.072	5.357
Rmeas	0.445	0.075	5.461
Rpim	0.088	0.017	1.051
Number of reflections	13344	666	668
<I/σ(I)>	10.5
Completeness [%]	94.3	99.6	58.1
Redundancy	25.5	19.9	26.7
CC(1/2)	0.997	0.997	0.270

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	magnesium chloride, sodium cacodilate, 2-methyl-2,4-pentandiol