6SJA

Structure of HPV16 E6 oncoprotein in complex with IRF3 LxxLL motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-27
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	97.659, 132.870, 43.025
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.830 - 1.500
R-factor	0.1606
Rwork	0.159
R-free	0.19050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4giz
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.832	45.832	1.590
High resolution limit [Å]	1.500	4.480	1.500
Rmerge	0.068	0.026	1.150
Rmeas	0.074	0.028	1.247
Total number of observations	1192946
Number of reflections	170435	6514	26948
<I/σ(I)>	15.95	61.58	1.65
Completeness [%]	98.1	99.5	95.7
Redundancy	6.999	7.595	6.704
CC(1/2)	0.999	0.999	0.618

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.8	298	PEG 1500 30%