6SH7
Crystal structure of the human DEAH-helicase DHX15 in complex with the NKRF G-patch
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000041 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 82.243, 90.024, 213.885 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.230 - 2.210 |
| R-factor | 0.2111 |
| Rwork | 0.209 |
| R-free | 0.25080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xdr |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.472 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.230 | 2.290 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmeas | 0.187 | |
| Rpim | 0.052 | 0.763 |
| Number of reflections | 39975 | 3912 |
| <I/σ(I)> | 8.2 | |
| Completeness [%] | 99.8 | 98.7 |
| Redundancy | 13.1 | 13.5 |
| CC(1/2) | 0.998 | 0.380 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291.15 | 0.1 M carboxylic acids, 0.1 M BICINE/Tris pH 8.5, 12.5% w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v MPD |
