6SG4
Structure of CDK2/cyclin A M246Q, S247EN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.309, 137.853, 109.774 |
| Unit cell angles | 90.00, 99.81, 90.00 |
Refinement procedure
| Resolution | 85.100 - 2.430 |
| R-factor | 0.20326 |
| Rwork | 0.201 |
| R-free | 0.25075 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jst |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.381 |
| Data reduction software | DIALS |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.100 | 2.520 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Number of reflections | 44281 | 4672 |
| <I/σ(I)> | 12 | 1.3 |
| Completeness [%] | 99.6 | 98.7 |
| Redundancy | 1.9 | 1.9 |
| CC(1/2) | 0.997 | 0.528 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293.15 | 200 mM K/Na Tartrate, 20 % PEG 3350 |
