6SFF

mouse Interleukin-12 subunit beta - p80 homodimer in space group I41

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	I 41
Unit cell lengths	85.876, 85.876, 107.671
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.140 - 2.400
R-factor	0.2329
Rwork	0.229
R-free	0.26450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mj3
RMSD bond length	0.003
RMSD bond angle	0.567
Data reduction software	XDS (20190315)
Data scaling software	XDS (20190315)
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	67.410	67.410	2.550
High resolution limit [Å]	2.400	7.170	2.400
Rmeas	0.125	0.037	1.551
Number of reflections	15233	592	2419
<I/σ(I)>	12.23	47.82	0.97
Completeness [%]	99.7	100	98.5
Redundancy	8.11	8.15	7.16
CC(1/2)	0.998	1.000	0.555

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	287	120 mM monosaccharides, 50 mM Imidazole, 50 mM MES, pH6.5, 20% Ethylene glycol, 10 % PEG 8000 (MORPHEUS 1, F2)