6SF3

Bone morphogenetic protein 10 (BMP10) in complex with extracellular domain of activin receptor-like kinase 1 (ALK1) at 2.3 Angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-19
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.976
Spacegroup name	P 65 2 2
Unit cell lengths	58.269, 58.269, 311.435
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	51.906 - 2.300
R-factor	0.215492606269
Rwork	0.214
R-free	0.24325
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6sf1
RMSD bond length	0.007
RMSD bond angle	0.850
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	311.440	2.380
High resolution limit [Å]	2.300	2.300
Rpim	0.075
Number of reflections	15103	1408
<I/σ(I)>	7.4	1.6
Completeness [%]	99.9	99.8
Redundancy	14.7	15.1
CC(1/2)	0.994	0.635

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	0.07 M Amino acids (0.014 M DL-Glutamic acids monohydrate; 0.014 M DL-Alanine; 0.014 M Glycine; 0.014 M DL-Lysine monohydrochloride; 0.014 M DL-Serine), 0.07 M Buffer System 3 (0.07 M Tris(base)/BICINE), 35% Precipitant Mix 4 (9% v/v MPD, 9% w/v PEG 1000, 9% w/ PEG3350)