6SES
Tubulin-B2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-27 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.99987 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.050, 157.170, 178.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.400 - 2.000 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5lxt |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.400 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.195 | 5.321 |
| Rmeas | 0.203 | 5.537 |
| Rpim | 0.062 | 1.537 |
| Number of reflections | 196846 | 14328 |
| <I/σ(I)> | 10.8 | |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 13.5 | 12.9 |
| CC(1/2) | 0.999 | 0.189 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.7 | 293 | 5% PEG 4K, 9% GLYCEROL, 30 MM MGCL2, 30 MM CACL2, 0.1 M MES/IMIDAZOLE PH 6.7 |
