6SEJ
Structure of a functional monomeric properdin lacking TSR3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | I 41 |
Unit cell lengths | 222.400, 222.400, 47.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.730 - 3.501 |
R-factor | 0.2434 |
Rwork | 0.242 |
R-free | 0.26640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | electron density for TB-TSR1-TSR5-TSR6 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.090 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.590 |
High resolution limit [Å] | 3.500 | 3.500 |
Number of reflections | 15199 | 1113 |
<I/σ(I)> | 9.2 | |
Completeness [%] | 99.8 | |
Redundancy | 6.6 | |
CC(1/2) | 0.990 | 0.192 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 292 | 1.0 M lithium sulfate, 0.1 M sodium acetate pH 4.0, 0.1 M barium chloride |