6SEJ

Structure of a functional monomeric properdin lacking TSR3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9794
Spacegroup name	I 41
Unit cell lengths	222.400, 222.400, 47.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.730 - 3.501
R-factor	0.2434
Rwork	0.242
R-free	0.26640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	electron density for TB-TSR1-TSR5-TSR6
RMSD bond length	0.007
RMSD bond angle	1.090
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.590
High resolution limit [Å]	3.500	3.500
Number of reflections	15199	1113
<I/σ(I)>	9.2
Completeness [%]	99.8
Redundancy	6.6
CC(1/2)	0.990	0.192

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	292	1.0 M lithium sulfate, 0.1 M sodium acetate pH 4.0, 0.1 M barium chloride