6S9I

Human Brr2 Helicase Region D534C/N1866C in complex with C-tail deleted Jab1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-18
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.91677
Spacegroup name	P 21 21 21
Unit cell lengths	99.240, 119.077, 187.913
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.976 - 2.598
R-factor	0.1991
Rwork	0.197
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.760
High resolution limit [Å]	2.590	2.600
Rmeas	0.159	1.386
Number of reflections	68538	10962
<I/σ(I)>	9.6	1.3
Completeness [%]	98.9	99.2
Redundancy	5.7
CC(1/2)	0.997	0.474

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291.15	0.1M Hepes-NaOH, 0.1M MgCl2, 8% PEG 3350