6S9E
Tubulin-GDP.AlF complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.999, 157.357, 180.261 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.003 - 2.250 |
| R-factor | 0.2041 |
| Rwork | 0.203 |
| R-free | 0.22780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o2b |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.526 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.003 | 48.000 | 2.290 |
| High resolution limit [Å] | 2.250 | 12.320 | 2.250 |
| Rmerge | 0.075 | 0.033 | 1.222 |
| Rmeas | 0.080 | 0.035 | 1.295 |
| Rpim | 0.025 | 0.012 | 0.417 |
| Total number of observations | 8884 | 62409 | |
| Number of reflections | 140201 | 982 | 6786 |
| <I/σ(I)> | 16.5 | 54.5 | 1.8 |
| Completeness [%] | 99.0 | 98.5 | 97.8 |
| Redundancy | 9.6 | 9 | 9.2 |
| CC(1/2) | 0.979 | 0.999 | 0.635 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.1M MES, 0.1M IMIDAZOLE, pH6.5, 0.03M CaCl2, 0.03M MgCl2, 5mM L-Tyrosine, 5% Glycerol, 5.5% PEG4000 |
