6S8Y
Crystal structure of cytochrome c in complex with a sulfonated quinoline-derived foldamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-09 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.98009 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 39.138, 39.138, 187.366 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.900 - 2.090 |
| R-factor | 0.227 |
| Rwork | 0.225 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lyc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.520 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.450 | 2.120 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.082 | 1.241 |
| Rmeas | 0.084 | 1.273 |
| Rpim | 0.020 | 0.283 |
| Number of reflections | 10590 | 10386 |
| <I/σ(I)> | 19 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 18.8 | |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 5 % PEG 3350, 100 mM sodium acetate pH 4.5, 200 mM zinc acetate |
