6S8O
Human Brr2 Helicase Region M641C/A1582C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918400 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 145.564, 152.531, 142.515 |
| Unit cell angles | 90.00, 120.69, 90.00 |
Refinement procedure
| Resolution | 48.383 - 3.172 |
| R-factor | 0.2666 |
| Rwork | 0.264 |
| R-free | 0.31840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f91 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.360 |
| High resolution limit [Å] | 3.170 | 3.170 |
| Rmeas | 0.121 | 1.316 |
| Number of reflections | 44087 | 6492 |
| <I/σ(I)> | 8.7 | |
| Completeness [%] | 97.0 | 89.3 |
| Redundancy | 3.4 | |
| CC(1/2) | 0.998 | 0.641 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291.15 | 0.1M sodium citrate, 1.5M sodium malonate |
