6S6D

Crystal structure of RagA-Q66L-GTP/RagC-S75N-GDP GTPase heterodimer complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	71.504, 81.439, 246.033
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.290 - 2.500
R-factor	0.2127
Rwork	0.209
R-free	0.28660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.704
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.290	49.290	2.650
High resolution limit [Å]	2.500	7.420	2.500
Rmerge	0.070	0.033	1.047
Rmeas	0.077	0.036	1.136
Total number of observations	331756
Number of reflections	50589	2092	8018
<I/σ(I)>	16.69	45.88	1.94
Completeness [%]	99.8	98.7	99
Redundancy	6.558	5.576	6.671
CC(1/2)	0.999	0.998	0.654

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100mM MOPSO, Bis-Tris pH 6.5, 40mM polyamines ( mixture of spermine tetrahydrochloride, spermidine trihydrochloride, 1,4. diaminobutane dihydrochloride, 0.1 M D/L-ornithine monohydrochloride), 10% (w/v) PEG 8000. 20% (v/v) 1,5 - Pentanediol and 24 % (v/v) glycerol.