6S6A

Crystal structure of RagA-Q66L/RagC-T90N GTPase heterodimer complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-21
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 21 21 21
Unit cell lengths	72.103, 81.856, 244.076
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.620 - 2.630
R-factor	0.1992
Rwork	0.195
R-free	0.27460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.850
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.620	46.620	2.790
High resolution limit [Å]	2.630	7.790	2.630
Rmerge	0.065	0.028	1.260
Rmeas	0.070	0.030	1.362
Total number of observations	292679
Number of reflections	43123	1801	6810
<I/σ(I)>	17.91	54.06	1.24
Completeness [%]	98.0	96.8	97.6
Redundancy	6.787	6.152	6.886
CC(1/2)	0.999	0.999	0.587

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	100mM MOPSO, Bis-Tris pH 6.5, 40mM polyamines ( mixture of spermine tetrahydrochloride, spermidine trihydrochloride, 1,4. diaminobutane dihydrochloride, 0.1 M D/L-ornithine monohydrochloride), 10% (w/v) PEG 8000. 20% (v/v) 1,5 - Pentanediol and 24 % (v/v) glycerol.