6RV2

Crystal structure of the human two pore domain potassium ion channel TASK-1 (K2P3.1) in a closed conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	P 2 21 21
Unit cell lengths	45.140, 204.790, 238.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.040 - 3.000
R-factor	0.254
Rwork	0.253
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bw5
RMSD bond length	0.008
RMSD bond angle	0.900
Data reduction software	XDS (VERSION Jan 26, 2018)
Data scaling software	XSCALE (VERSION Mar 15, 2019)
Phasing software	PHASER (2.7.17)
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.668	3.120
High resolution limit [Å]	3.000	3.000
Rmerge	0.215	1.021
Rmeas	0.239	1.124
Rpim	0.103	0.464
Number of reflections	36417	1822
<I/σ(I)>	5.6	1.9
Completeness [%]	79.7	37.2
Redundancy	5.3	5.7
CC(1/2)	0.993	0.697

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.1 M TRIS pH 8.5, 0.05 M KCl, 32% v/v PEG400, 3% w/v sucrose