6RT5
The YTH domain of YTHDC1 protein in complex with Gm6AC oligonucleotide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-29 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.000002 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.787, 103.655, 41.779 |
Unit cell angles | 90.00, 104.76, 90.00 |
Refinement procedure
Resolution | 40.400 - 2.303 |
R-factor | 0.2118 |
Rwork | 0.209 |
R-free | 0.27480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4r3h |
RMSD bond length | 0.007 |
RMSD bond angle | 0.911 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.410 | 2.440 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmeas | 0.043 | 0.147 |
Number of reflections | 14246 | 2119 |
<I/σ(I)> | 26.26 | |
Completeness [%] | 97.8 | 91.1 |
Redundancy | 4.48 | 4.5 |
CC(1/2) | 0.999 | 0.988 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 298 | 0.1 M Bis-Tris at pH 6.5, 0.2 M ammonium sulfate and 30% PEG 3350 |