6RPT
Structure of tick complement inhibitor CirpT1 complexed with macroglobubulin domain 4 of human complement C5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.830, 56.948, 90.072 |
| Unit cell angles | 90.00, 113.02, 90.00 |
Refinement procedure
| Resolution | 82.900 - 2.700 |
| Rwork | 0.226 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hcc |
| Data reduction software | DIALS (xia2 Dials) |
| Data scaling software | DIALS (xia2 Dials) |
| Phasing software | MOLREP (CCP4Interface 7.0.056) |
| Refinement software | PHENIX (dev_3126) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.900 | 2.560 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmerge | 0.266 | |
| Number of reflections | 22552 | 2243 |
| <I/σ(I)> | 3.8 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 284.15 | 0.02 M Na2PO4/K2PO4, 20 % w/v PEG3350 |
