6RP5
Crystal structure of monocarboxylated hemoglobin from the sub-Antarctic fish Eleginops maclovinus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-07 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 91.702, 91.702, 168.716 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 79.420 - 1.490 |
| R-factor | 0.16974 |
| Rwork | 0.168 |
| R-free | 0.19577 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4esa |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.136 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.420 | 1.530 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.095 | 0.440 |
| Rpim | 0.057 | 0.295 |
| Number of reflections | 66710 | 5202 |
| <I/σ(I)> | 8 | |
| Completeness [%] | 96.4 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 277 | CO atmosphere , 20 mg/ml protein, 1.8 M ammonium sulfate |
