6RJP

Bfl-1 in complex with alpha helical peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-31
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.975312
Spacegroup name	P 31 2 1
Unit cell lengths	113.749, 113.749, 79.148
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.570
R-factor	0.2025
Rwork	0.199
R-free	0.26590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vm6
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.700	2.940
High resolution limit [Å]	2.570	2.570
Rmerge	0.046	0.685
Rmeas	0.051	0.776
Rpim	0.027	0.359
Number of reflections	10669	533
<I/σ(I)>	13.3
Completeness [%]	55.5
Redundancy	3.6
CC(1/2)	0.999	0.692

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	0.1 M sodium acetate pH 4.6 16% (w/v) PEG smear high