6RFP

ERK2 MAP kinase with mutations at Helix-G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-05
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.972
Spacegroup name	P 1 21 1
Unit cell lengths	48.740, 68.810, 60.810
Unit cell angles	90.00, 110.14, 90.00

Refinement procedure

Resolution	45.800 - 1.740
R-factor	0.14652
Rwork	0.144
R-free	0.19726
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4s31
RMSD bond length	0.016
RMSD bond angle	1.967
Data reduction software	MOSFLM
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.800	1.790
High resolution limit [Å]	1.740	1.740
Number of reflections	38438	2840
<I/σ(I)>	16.1
Completeness [%]	99.4
Redundancy	3.7
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.8 M ammonium sulfate 0.1 M Bis-tris pH 6.8 or 6.5 2% (w/v) PEG 550