6RD0
Human MMP12 catalytic domain in complex with AP280
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-15 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.540 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 69.430, 62.580, 37.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.480 - 1.900 |
| R-factor | 0.1688 |
| Rwork | 0.167 |
| R-free | 0.20885 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rmz |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.336 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.480 | 2.010 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.110 | 0.800 |
| Number of reflections | 13391 | 2079 |
| <I/σ(I)> | 22.3 | 2.6 |
| Completeness [%] | 99.5 | 98 |
| Redundancy | 13.4 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 25% PEH 6000, 0.1 M Tris-HCl |
