6RCN

Crystal structure of REXO2-D199A-dAdA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.2824
Spacegroup name	C 2 2 21
Unit cell lengths	35.840, 126.890, 168.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.750 - 2.250
R-factor	0.213
Rwork	0.212
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.010
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	21.750	21.750	2.380
High resolution limit [Å]	2.250	6.670	2.250
Rmerge	0.071	0.038	1.390
Rmeas	0.077	0.041	1.504
Total number of observations	241414
Number of reflections	35196	1349	5631
<I/σ(I)>	14.15	46.58	1.17
Completeness [%]	99.7	99.4	98.6
Redundancy	6.859	6.719	6.807
CC(1/2)	0.999	0.999	0.536

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	100 mM Hepes pH 7.0, 200 mM sodium malonate, and 20 % polyethylene glycol 3350