6RA9

Novel structural features and post-translational modifications in eukaryotic elongation factor 1A2 from Oryctolagus cuniculus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	80
Detector technology	PIXEL
Collection date	2015-11-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979260
Spacegroup name	P 61 2 2
Unit cell lengths	133.520, 133.520, 305.440
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	57.820 - 2.700
R-factor	0.1811
Rwork	0.179
R-free	0.22410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4c0s
RMSD bond length	0.003
RMSD bond angle	0.635
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.170	2.790
High resolution limit [Å]	2.700	2.700
Rmerge	0.120	1.069
Rmeas	0.125	1.118
Rpim	0.034	0.320
Number of reflections	83606	4339
<I/σ(I)>	12.3
Completeness [%]	100.0	100
Redundancy	13	11.2
CC(1/2)	0.995	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	50 mM Na citrate pH 5/MES pH 5.6, 2.2-2.6 M ammonium sulphate, 10 mM Mg acetate