6RA4

Human ARGONAUTE-2 PAZ DOMAIN (214-347) IN COMPLEX WITH CGUGACUCU

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-30
Detector	MARRESEARCH
Wavelength(s)	0.99990
Spacegroup name	P 21 21 2
Unit cell lengths	72.617, 87.672, 56.832
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.890 - 1.900
Rwork	0.239
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.300
Data reduction software	HKL-2000 (1.98.7)
Data scaling software	SCALEPACK (1.98.7)
Phasing software	PHASER
Refinement software	CNS (2002)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.042	0.504
Total number of observations	213970
Number of reflections	29356	3009
<I/σ(I)>	48	3.29
Completeness [%]	99.1	99
Redundancy	7.3	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	292	Drop was a 1:1 mix of protein stock (9.4MG/ML HAGO2 PAZ [214 TO 347] IN 100MM POTASSIUM CHLORIDE, 10MM DTT, 5MM HEPES/KOH PH 7.6, WITH A 1.1-FOLD EXCESS OF OLIGONUCLEOTIDE ADDED FROM A 1.75MM STOCK SOLUTION IN 100MM POTASSIUM CHLORIDE, 10MM DTT, 5MM HEPES) and reservoir solution (0.2M sodium CHLORIDE 20% PEG 3,350)