6R8X
COAGULATION FACTOR XI CATALYTIC DOMAIN IN COMPLEX WITH FAB-PORTION OF MAA868
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-18 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.00002 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 191.274, 53.216, 65.164 |
| Unit cell angles | 90.00, 94.56, 90.00 |
Refinement procedure
| Resolution | 64.900 - 2.040 |
| Rwork | 0.220 |
| R-free | 0.28220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | fXI CD plus trucated Fab structures |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.960 | 2.090 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.099 | 0.571 |
| Number of reflections | 40205 | |
| <I/σ(I)> | 10.87 | 2.67 |
| Completeness [%] | 95.9 | 98.4 |
| Redundancy | 3.22 | 3.18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20% PEG 3350 9MG/ML SEEDING (ORIGINAL CRYSTAL FROM WHICH SEEDS WERE PREPARED GREW AFTER 3 WEEKS) |
