6R5E

Crystal structure of the Pri1 subunit of human primase bound to 2F-ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03978
Spacegroup name	C 2 2 21
Unit cell lengths	110.500, 117.180, 152.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.410 - 1.850
R-factor	0.1797
Rwork	0.178
R-free	0.20570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rb4
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260.0000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.410	1.960
High resolution limit [Å]	1.850	1.850
Number of reflections	82430	12971
<I/σ(I)>	11.52	1.52
Completeness [%]	97.7	95.8
Redundancy	4.39
CC(1/2)	0.998	0.773

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	23% PEG3350, 10% ethylene glycol, 200 mM Na/K tartrate