6R4L
Crystal structure of S. cerevisia Niemann-Pick type C protein NCR1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97950 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 148.670, 90.060, 161.680 |
Unit cell angles | 90.00, 110.31, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.124 | 49.124 | 3.400 |
High resolution limit [Å] | 3.300 | 20.000 | 3.300 |
Rmerge | 0.066 | 0.011 | 2.147 |
Rmeas | 0.089 | 0.016 | 2.892 |
Number of reflections | 28581 | 108 | 2449 |
<I/σ(I)> | 7.05 | 50.39 | 0.44 |
Completeness [%] | 93.9 | 68.8 | 95.7 |
Redundancy | 1.802 | 1.787 | 1.814 |
CC(1/2) | 0.999 | 1.000 | 0.172 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 6.1 | 291 | MES, Ammonium Chloride, Manganese Chloride, PEP 426 |