6R2N

Crystal structure of KlGlk1 glucokinase from Kluyveromyces lactis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	C 2 2 21
Unit cell lengths	102.011, 122.771, 360.346
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.077 - 2.596
R-factor	0.2051
Rwork	0.204
R-free	0.24310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3o8m
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.080	2.689
High resolution limit [Å]	2.596	2.596
Rmerge	0.046	0.482
Rmeas	0.065	0.681
Rpim	0.046	0.482
Number of reflections	69999	6782
<I/σ(I)>	13.6	1.7
Completeness [%]	99.6	97.4
Redundancy	2	2
CC(1/2)	0.986	0.528

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.15 M KBr and 30 % PEG 2000 MME