6R12
Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with compound 7b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.417, 58.795, 88.234 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.930 - 1.740 |
| R-factor | 0.17703 |
| Rwork | 0.174 |
| R-free | 0.23398 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v2y |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.259 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.930 | 1.840 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.140 | 0.890 |
| Number of reflections | 30720 | 54861 |
| <I/σ(I)> | 10.55 | |
| Completeness [%] | 98.8 | 92.4 |
| Redundancy | 12.46 | 11.99 |
| CC(1/2) | 1.000 | 0.860 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.4 M Ammonium phosphate |
