6QZK

Structure of Clostridium butyricum Argonaute bound to a guide DNA (5' deoxycytidine) and a 19-mer target DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-16
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00
Spacegroup name	P 63 2 2
Unit cell lengths	181.450, 181.450, 142.990
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	76.606 - 3.548
R-factor	0.2501
Rwork	0.247
R-free	0.27590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gq9
RMSD bond length	0.004
RMSD bond angle	0.720
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.606	3.675
High resolution limit [Å]	3.548	3.548
Rmerge	0.281	1.876
Rmeas	0.284	1.907
Number of reflections	17344	1696
<I/σ(I)>	17.05	2.47
Completeness [%]	99.9	99.94
Redundancy	39	38
CC(1/2)	0.999	0.861

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	NaForm, Nickel chloride