6QWS
Crystal structure of the Ski2 RNA-helicase Brr2 from Chaetomium thermophilum in the apo state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-30 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 31 |
| Unit cell lengths | 124.520, 124.520, 128.020 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.630 - 3.300 |
| R-factor | 0.2096 |
| Rwork | 0.206 |
| R-free | 0.26150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5m59 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.568 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.390 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmeas | 0.270 | |
| Number of reflections | 33329 | 2454 |
| <I/σ(I)> | 7.38 | 1.16 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 4.4 | |
| CC(1/2) | 0.980 | 0.370 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | Tris-HCl, PEG 3350, lithium sulfate |
