6QUP
Structural signatures in EPR3 define a unique class of plant carbohydrate receptors
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.822, 36.083, 72.661 |
| Unit cell angles | 90.00, 93.92, 90.00 |
Refinement procedure
| Resolution | 72.491 - 1.871 |
| R-factor | 0.1666 |
| Rwork | 0.163 |
| R-free | 0.21020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ebz |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.491 | 1.940 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.087 | 0.820 |
| Number of reflections | 25177 | 2151 |
| <I/σ(I)> | 15.96 | 1.94 |
| Completeness [%] | 96.9 | 81.1 |
| Redundancy | 6.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 292 | 0.1 M Sodium Citrate pH 5.5, 20% PEG 4000, 18% 2-propanol |
