6QTN

Tubulin-cyclostreptin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-27
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	104.585, 158.361, 179.946
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.655 - 1.900
R-factor	0.188
Rwork	0.187
R-free	0.21330
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
RMSD bond length	0.004
RMSD bond angle	0.652
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.700	1.950
High resolution limit [Å]	1.900	1.900
Rmerge	0.102	4.485
Rmeas	0.104	4.572
Rpim	0.025	0.843
Number of reflections	234346	17147
<I/σ(I)>	22.7	0.9
Completeness [%]	100.0	100
Redundancy	26.8	27
CC(1/2)	1.000	0.322

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.7	293	6% glycerol, 3% PEG4000, 0.1 M Mes/0.1 M imidazole pH 6.7, 30 mM CaCl2, 30 mM MgCl2