6QSK
Crystal structure of a nucleotide sugar transporter with bound nucleotide monophosphate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 83 |
| Detector technology | PIXEL |
| Collection date | 2017-09-30 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98010 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.705, 102.716, 181.253 |
| Unit cell angles | 89.93, 90.08, 90.19 |
Refinement procedure
| Resolution | 49.428 - 3.394 |
| R-factor | 0.2559 |
| Rwork | 0.253 |
| R-free | 0.31960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5oge |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.381 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_3360: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.430 | 3.520 |
| High resolution limit [Å] | 3.390 | 3.390 |
| Rmerge | 0.100 | 0.680 |
| Rmeas | 0.135 | 0.920 |
| Rpim | 0.090 | 0.610 |
| Number of reflections | 44054 | 4492 |
| <I/σ(I)> | 5.4 | 1.2 |
| Completeness [%] | 96.7 | 92.3 |
| Redundancy | 2 | 2 |
| CC(1/2) | 0.997 | 0.523 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 5 | 277 | 26 - 30 % (v/v) PEG 400, 0.1 M sodium citrate pH 5.0 and 75 mM sodium chloride or sodium acetate. GMP was soaked in at 20mM overnight. |
