6QPG
Influenza A virus Polymerase Heterotrimer A/nt/60/1968(H3N2) in complex with Nanobody NB8205
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 335.181, 192.900, 235.096 |
| Unit cell angles | 90.00, 91.78, 90.00 |
Refinement procedure
| Resolution | 234.980 - 3.340 |
| R-factor | 0.254 |
| Rwork | 0.251 |
| R-free | 0.30490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5d98 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.660 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 235.000 | 3.870 |
| High resolution limit [Å] | 3.340 | 3.340 |
| Number of reflections | 89646 | 4718 |
| <I/σ(I)> | 4.8 | 1.8 |
| Completeness [%] | 41.5 | 6.2 |
| Redundancy | 7 | 6.9 |
| CC(1/2) | 0.990 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 0.9-1.2M Phosphate Potassium Sodium, pH6.7 |
