6QP9
Drosophila Semaphorin 1a, extracellular domains 1-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-27 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 32 |
| Unit cell lengths | 99.476, 99.476, 150.131 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.738 - 3.600 |
| R-factor | 0.2934 |
| Rwork | 0.287 |
| R-free | 0.32260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fkk |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.575 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.740 | 3.820 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.233 | |
| Number of reflections | 19133 | |
| <I/σ(I)> | 5 | |
| Completeness [%] | 97.0 | |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | 0.1 M Tris-HCl (pH 8.0), 0.2 M ammonium sulphate, 25% PEG 3350 |
