6QNB

Liquid Application Method for time-resolved Analyses (LAMA) by serial synchrotron crystallography, Lysozyme with GlcNAc3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	293
Detector technology	PIXEL
Collection date	2018-09-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	P 43 21 2
Unit cell lengths	79.210, 79.210, 38.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.802 - 1.700
R-factor	0.1946
Rwork	0.194
R-free	0.21080
RMSD bond length	0.006
RMSD bond angle	0.834
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.802	1.761
High resolution limit [Å]	1.700	1.700
Number of reflections	13934
<I/σ(I)>	8.8
Completeness [%]	99.9
Redundancy	148.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293	20 mM NaAcetate pH 4.7, 50 mM NaCl