6QLB

Calpain small subunit 1, RNA-binding protein Hfq

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 3
Unit cell lengths	147.607, 147.607, 147.607
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.010 - 2.320
R-factor	0.20328
Rwork	0.201
R-free	0.24740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4phj
RMSD bond length	0.013
RMSD bond angle	1.855
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.020	2.380
High resolution limit [Å]	2.320	2.320
Rmerge	0.114	1.115
Rmeas	0.116	1.141
Number of reflections	46565	3416
<I/σ(I)>	19.2
Completeness [%]	100.0	100
Redundancy	22.1	22.2
CC(1/2)	0.999	0.632

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293	Molecular Dimensions Pact premier screen condition D06: 0.1 M MMT, 25% PEG1500, pH 9.0