6QL7

Structure of fatty acid synthase complex with bound gamma subunit from Saccharomyces cerevisiae at 4.6 angstrom

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976
Spacegroup name	P 1 21 1
Unit cell lengths	234.922, 430.307, 422.610
Unit cell angles	90.00, 97.01, 90.00

Refinement procedure

Resolution	192.500 - 4.600
R-factor	0.2545
Rwork	0.252
R-free	0.30980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	1.226
Data reduction software	XDS
Data scaling software	Aimless (0.7.3)
Phasing software	MOLREP (11.6.04)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	192.500	4.700
High resolution limit [Å]	4.600	4.600
Rpim	0.088	0.829
Number of reflections	458021	45686
<I/σ(I)>	7.95	0.87
Completeness [%]	99.5	99.71
Redundancy	7.2	7.4
CC(1/2)	0.998	0.364

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	291	0.1 M MES pH 6.2, 0.15-0.3 M ammonium sulphate, 7-8% (w/v) PEG 8000