6QK8

Crystal structure of yeast 14-3-3 protein (Bmh1) from Saccharomyces cerevisiae with the Nha1p (yeast Na+/H+ antiporter) 14-3-3 binding motif Ser481

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2018-11-29
Detector	RAYONIX MX225HE
Wavelength(s)	0.9184
Spacegroup name	P 1
Unit cell lengths	56.850, 59.112, 66.700
Unit cell angles	95.90, 104.14, 91.19

Refinement procedure

Resolution	41.141 - 2.917
R-factor	0.2204
Rwork	0.218
R-free	0.27080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n6n
RMSD bond length	0.004
RMSD bond angle	0.511
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.141	3.022
High resolution limit [Å]	2.917	2.917
Rmeas	0.167	0.617
Number of reflections	16913	1720
<I/σ(I)>	7.23	2.15
Completeness [%]	92.8	94.51
Redundancy	2.98	4.81
CC(1/2)	0.986	0.723

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293.15	KBr, PEG 2K MME