6QF6

Structure of an E.coli expressed anti-Mcl1 scFv

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-24
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9763
Spacegroup name	P 43 21 2
Unit cell lengths	180.175, 180.175, 88.417
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	127.400 - 2.590
Rwork	0.181
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qb3
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	127.403	3.010
High resolution limit [Å]	2.590	2.590
Number of reflections	23417	26817
<I/σ(I)>	5.75
Completeness [%]	91.3
Redundancy	12.92
CC(1/2)	0.978	0.369

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.4 M Ammonium Sulphate, 25% PEG 3350, 0.1 M BIS-TRIS pH 5.9