6QF5
X-Ray structure of human Aquaporin 2 crystallized on a silicon chip
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SLAC LCLS BEAMLINE MFX |
Synchrotron site | SLAC LCLS |
Beamline | MFX |
Temperature [K] | 298 |
Detector technology | PIXEL |
Collection date | 2016-11-13 |
Detector | CS-PAD XPP |
Wavelength(s) | 1.3670 |
Spacegroup name | P 42 |
Unit cell lengths | 122.200, 122.200, 94.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.892 - 3.700 |
R-factor | 0.2873 |
Rwork | 0.282 |
R-free | 0.33570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nef |
RMSD bond length | 0.004 |
RMSD bond angle | 0.761 |
Data reduction software | CrystFEL |
Data scaling software | CrystFEL |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1-2155_9999) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.380 | 3.760 |
High resolution limit [Å] | 3.700 | 3.700 |
Number of reflections | 14866 | 1462 |
<I/σ(I)> | 2.32 | 0.92 |
Completeness [%] | 100.0 | 99.93 |
Redundancy | 28.1 | 17.7 |
CC(1/2) | 0.783 | 0.105 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 0.1M Tris pH 8.5, 0.1M sodium chloride, 0.1M magnesium chloride, 22-25% PEG 400, cadmium chloride |