6QEI

CRYSTAL STRUCTURE OF HUMAN METHIONINE AMINOPEPTIDASE-2 IN COMPLEX WITH AN INHIBITOR 5,6-Difluoro-3-(2-isopropoxy-4-piperazin-1-yl-phenyl)-1H-indole-2-carboxylic acid amide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000000
Spacegroup name	C 2 2 21
Unit cell lengths	89.808, 99.784, 100.819
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.460 - 1.800
R-factor	0.19
Rwork	0.189
R-free	0.20900
RMSD bond length	0.010
RMSD bond angle	1.000
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.500	1.910
High resolution limit [Å]	1.800	1.800
Rmeas	0.136	2.327
Number of reflections	42203	6703
<I/σ(I)>	11.8	0.88
Completeness [%]	99.9	99.3
Redundancy	6.6	6.4
CC(1/2)	0.998	0.458

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	20% METHANOL, 0.1 M CITRATE, PH 6.0,