6QBB
Engineered streptavidin variant (ENAGY) in complex with the Strep-tag II peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 45.560, 64.170, 78.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.580 - 1.520 |
| R-factor | 0.16295 |
| Rwork | 0.162 |
| R-free | 0.19021 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rst |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.483 |
| Data reduction software | MOSFLM (7.1.0) |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.580 | 1.600 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.076 | 0.342 |
| Rmeas | 0.085 | 0.386 |
| Rpim | 0.039 | 0.176 |
| Number of reflections | 35992 | 5162 |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG MME 550, zinc sulfate, 2-(N-morpholino)ethanesulfonic acid |
