6QB4

Mcl1-scFv complex with an indole acid inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-14
Detector	ADSC QUANTUM 315
Wavelength(s)	0.92
Spacegroup name	C 1 2 1
Unit cell lengths	144.947, 40.861, 77.354
Unit cell angles	90.00, 111.42, 90.00

Refinement procedure

Resolution	39.110 - 2.380
R-factor	0.198
Rwork	0.197
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.060
Data reduction software	MOSFLM
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.150	2.510
High resolution limit [Å]	2.380	2.380
Number of reflections	16889
<I/σ(I)>	7.7
Completeness [%]	98.5
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PCPT 0.1M pH 7.5, PEG 3350 15%w/v, MgCl2 0.1M