6QAT
Crystal structure of ULK2 in complexed with hesperadin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.208, 77.561, 94.941 |
| Unit cell angles | 90.00, 97.77, 90.00 |
Refinement procedure
| Resolution | 48.750 - 2.770 |
| R-factor | 0.21993 |
| Rwork | 0.218 |
| R-free | 0.26160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wno |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.098 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.750 | 2.920 |
| High resolution limit [Å] | 2.770 | 2.770 |
| Rmerge | 0.071 | 0.758 |
| Number of reflections | 27487 | 4001 |
| <I/σ(I)> | 10.1 | 2 |
| Completeness [%] | 99.2 | 99.3 |
| Redundancy | 5.1 | 5.1 |
| CC(1/2) | 0.997 | 0.884 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 27.5% PEG 3350, 0.1 M sodium citrate, pH 5.9, 0.15 M MgCl2, 0.1 M bis-tris, pH 5.75, 5% glycerol |
